Identification of QTLs associated with seed protein concentration in two diverse recombinant inbred line populations of pea
Identification of QTLs associated with seed protein concentration in two diverse recombinant inbred line populations of pea
Blog Article
Improving the seed protein concentration (copyright) of pea (Pisum sativum L.) has turned into an important breeding objective because of the consumer demand for plant-based protein and demand from protein fractionation industries.To support the marker-assisted selection (MAS) of copyright towards accelerated breeding of improved cultivars, we have explored two diverse recombinant inbred line (RIL) populations to identify the quantitative trait loci (QTLs) associated with copyright.The two RIL Copper Tube Flaring Kit populations, MP 1918 × P0540-91 (PR-30) and Ballet × Cameor (PR-31), were derived from crosses between moderate copyright × high copyright accessions.A total of 166 and 159 RILs of PR-30 and PR-31, respectively, were genotyped using an Axiom® 90K SNP array and 13.
2K SNP arrays, respectively.The RILs were phenotyped in replicated trials in two and three locations of Saskatchewan, copyright in 2020 and 2021, respectively, for agronomic assessment and copyright.Using composite interval mapping, we identified three QTLs associated with copyright in PR-30 and five QTLs in PR-31, with the LOD value ranging from 3.0 to 11.0.
A majority of these QTLs were unique to these populations compared to the previously known QTLs for copyright.The QTL copyright-Ps-5.1 overlapped with the earlier reported copyright associated QTL PC-QTL-3.Three QTLs, copyright-Ps-4.2, copyright-Ps-5.
1, and copyright-Ps-7.2 with LOD scores of 7.2, 7.9, and 11.3, and which explained 14.
5%, 11.6%, and 11.3% of the phenotypic variance, respectively, can be used for marker-assisted breeding to increase copyright in peas.Eight QTLs associated with the grain yield were identified with LOD scores ranging from 3.1 to 8.
2.Two sets of QTLs, copyright-Ps-2.1 and GY-Ps-2.1, and copyright-Ps-5.1 and Tennis Racquet GY-Ps-5.
3, shared the QTL/peak regions.Each set of QTLs contributed to either copyright or grain yield depending on which parent the QTL region is derived from, thus confirming that breeding for copyright should take into consideration the effects on grain yield.